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Nucleic Acids Research, 1991, Vol. 19, No. 14 3887-3891
© 1991

MOLECULAR BIOLOGY

K-tuple frequency in the human genome and polymerase chain reaction

R. Gnffais, P.M. André1 and M. Thibon

Laboratoire des Chlamydiales et des Rickettsiales, Institut Pasteur 28 rue du Docteur Roux, 75015 Pans 1Laboratoire de Bacténologie-Virologie, Faculté de Médecine 35043 Rennes Cedex, France

Received April 29, 1991. Revised July 1, 1991. Accepted July 1, 1991.

The frequency occurences of K-tuple (overlapping sequences of defined length, K) were computed from the known human genome sequences. The significance of these frequencies for the whole human genome was tested by polymerase chain reaction (PCR). A computer program based on these results was written to choose primers to amplify DNA target sequences, either of human genes or of human infectious agents. The software also gave nested primer sequences which were used to synthesize non radioactive probes by PCR. We applied these two methods, primer selection and non radioactive probes, to easily and quickly set up very efficient PCR sets to work in the human genome context.


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