Nucleic Acids Research, 1991, Vol. 19, No. 14 3901-3905
© 1991
MOLECULAR BIOLOGY |
The effect of the length of direct repeats and the presence of palindromes on deletion between directly repeated DNA sequences in bacteriophage T7
Department of Biochemistry and Molecular Biology, Temple University School of Medicine Philadelphia, PA 19140, USA
* To whom correspondence should be addressed
Received April 26, 1991. Revised June 19, 1991. Accepted June 19, 1991.
The frequency of genetic deletion between directly repeated DNA sequences in bacteriophage T7 was measured as a function of the length of the direct repeat. The non-essential ligase gene (gene 1.3) of bacteriophage T7 was Interrupted with pieces of synthetic DNA bracketed by direct repeats of various lengths. Deletion of these 76 bp long inserts was too low to be measured when the direct repeats were less than 6 bp long. However, the frequency of deletion of inserts with longer direct repeats increased exponentially as the length of the repeats increased from 8 to 20 bp. When Inverted repeats (palindromes) were designed in the midst of the insert there was essentially no increase In deletion frequency between 10 bp direct repeats. But, the same palindromic sequences increased the deletion frequency between 5 bp direct repeats by at least two orders of magnitude. Thus, in this system homology at the endpoints Is a more important determinant of deletion frequency than is the presence of palindromes between the direct repeats.
+ Present address: The E.I. DuPont Merck Pharmaceutical Co, Experimental Station, Wilmington, DE 19880-0328, USA
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