Nucleic Acids Research, 1991, Vol. 19, No. 18 4925-4929
© 1991
MOLECULAR BIOLOGY |
Regulatory regions of rat insulin I gene necessary for expression in transgenic mice
Institut Jacques Monod, Unité INSERM 257, Université Paris VII 75251 Paris Cedex 05, France
Received June 14, 1991. Accepted August 13, 1991.
Ten transgenic mouse lines harboring the –346/–103 fragment of the rat insulin I enhancer linked to a eterologous promoter and a reporter gene (Elns-Ptk-CAT construct) were produced. Expression of the hybrid transgene was essentially observed in pancreas and to a lesser extent in brain. These results Indicate that the rat insulin I promoter is dispensable for pancreatic expression. This insulin gene sequence is the shortest fragment described as conferring tissue-specific expression in transgenic mice. Two short homologous sequences in the rat insulin I enhancer fragment used, IEB2 and IEB1, have been described as playing a dominant role In the regulation of HIT hamster insulinoma cell-specific transcription of the insulin gene (1). We investigated whether the combination of IEB2 and IEB1 sequences is sufficient to confer specific expression in transgenic mice to a IEB2-IEB1-Ptk-CAT gene construct. No CAT activity was observed neither in pancreas nor In any other organ examined in 19 different transgenic mice. Moreover In transient expression experiments in RIN2A rat insulinoma cells, the IEB sequences had a very weak or no enhancer activity. These observations contribute to the conclusion that DNA regulatory elements other than the IEB sequences are necessary for gene expression in vivo.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. C. Martinez, K. Tanabe, C. Cras-Meneur, N. A. Abumrad, E. Bernal-Mizrachi, and M. A. Permutt Inhibition of Foxo1 Protects Pancreatic Islet {beta}-Cells Against Fatty Acid and Endoplasmic Reticulum Stress-Induced Apoptosis Diabetes, April 1, 2008; 57(4): 846 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Koster, M. S. Remedi, R. Masia, B. Patton, A. Tong, and C. G. Nichols Expression of ATP-Insensitive KATP Channels in Pancreatic {beta}-Cells Underlies a Spectrum of Diabetic Phenotypes Diabetes, November 1, 2006; 55(11): 2957 - 2964. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Le Lay and R. Stein Involvement of PDX-1 in activation of human insulin gene transcription J. Endocrinol., February 1, 2006; 188(2): 287 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Iype, J. Francis, J. C. Garmey, J. C. Schisler, R. Nesher, G. C. Weir, T. C. Becker, C. B. Newgard, S. C. Griffen, and R. G. Mirmira Mechanism of insulin Gene Regulation by the Pancreatic Transcription Factor Pdx-1: APPLICATION OF PRE-mRNA ANALYSIS AND CHROMATIN IMMUNOPRECIPITATION TO ASSESS FORMATION OF FUNCTIONAL TRANSCRIPTIONAL COMPLEXES J. Biol. Chem., April 29, 2005; 280(17): 16798 - 16807. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Alba, M. C. Puertas, J. Carrillo, R. Planas, R. Ampudia, X. Pastor, F. Bosch, R. Pujol-Borrell, J. Verdaguer, and M. Vives-Pi IFN{beta} Accelerates Autoimmune Type 1 Diabetes in Nonobese Diabetic Mice and Breaks the Tolerance to {beta} Cells in Nondiabetes-Prone Mice J. Immunol., December 1, 2004; 173(11): 6667 - 6675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Le Lay, T.-a. Matsuoka, E. Henderson, and R. Stein Identification of a Novel PDX-1 Binding Site in the Human Insulin Gene Enhancer J. Biol. Chem., May 21, 2004; 279(21): 22228 - 22235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Dumonteil, B. Laser, I. Constant, and J. Philippe Differential Regulation of the Glucagon and Insulin I Gene Promoters by the Basic Helix-Loop-Helix Transcription Factors E47 and BETA2 J. Biol. Chem., August 7, 1998; 273(32): 19945 - 19954. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pelegrin, J. C. Devedjian, C. Costa, J. Visa, G. Solanes, A. Pujol, G. Asins, A. Valera, and F. Bosch Evidence from Transgenic Mice That Interferon-beta May Be Involved in the Onset of Diabetes Mellitus J. Biol. Chem., May 15, 1998; 273(20): 12332 - 12340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M.M. Stellrecht, F. J. DeMayo, M. J. Finegold, and M.-J. Tsai Tissue-specific and Developmental Regulation of the Rat Insulin II Gene Enhancer, RIPE3, in Transgenic Mice J. Biol. Chem., February 7, 1997; 272(6): 3567 - 3572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Harrington and A. Sharma Transcription Factors Recognizing Overlapping C1-A2 Binding Sites Positively Regulate Insulin Gene Expression J. Biol. Chem., January 5, 2001; 276(1): 104 - 113. [Abstract] [Full Text] [PDF]
|
|