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Nucleic Acids Research, 1991, Vol. 19, No. 19 5321-5329
© 1991

MOLECULAR BIOLOGY

Adult chcken {alpha}-globin gene expression in transfected QT6 quail cells: evidence for a negative regulatory element in the {alpha}D gene region

Wynne Lewis, Jiing-Dwan Lee and Jerry B. Dodgson*

Department and Microbiology and Biochemistry, Michegan State University East Lansing, ML 48824, USA

*To whom correspondence should be addressed

Received June 26, 1991. Accepted September 4, 1991.

The chicken adult {alpha}-globin genes, {alpha}A and {alpha}D, are closely linked in chromosomal DNA and are coordinately expressed in vivo in an approximate 3:1 ratio, respectively. When subcloned DNAs containing one or the other gene are stably transfected into QT6 quail fibroblasts, the {alpha}A-globin gene is expressed at measurable RNA levels, but the {alpha}D gene is not. The {alpha}A gene expression can be considerably increased by the presence of a linked Rous sarcoma virus long terminal repeat enhancer, but that of the {alpha}D gene remains undetectable. Transfection with subclones containing both genes, either in cis or in trans, leads to considerably reduced {alpha}A RNA levels and still no observable {alpha}D gene expression. Transfection with deleted subclones suggests that maximal expression levels in this system require the {alpha}A-globin gene promoter, as opposed to that of the {alpha}D gene, but that such expression is greatly reduced by one or more DNA sequences which lie approximately 2,000 base pairs upstream of the {alpha}A gene, within the body of the {alpha}D gene.


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A. A. Gavrilov and S. V. Razin
Spatial configuration of the chicken {alpha}-globin gene domain: immature and active chromatin hubs
Nucleic Acids Res., August 1, 2008; 36(14): 4629 - 4640.
[Abstract] [Full Text] [PDF]


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