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Nucleic Acids Research, 1991, Vol. 19, No. 19 5339-5344
© 1991

MOLECULAR BIOLOGY

Domonstration of a divergent transcript from the bidirectional heavy chain immunoglobulin promoter VH441 in B-cells

Quang Tri Nguyen, Noëlle Doyen, Martine Fanton d'Andon and Francois Rougeon*

Institut Pasteur, Unitè de Gènètique et Biochimie du Dèvelopment, URA CNRS 361, Dèpartement d'lmmunologie 25 rue du Dr Roux, 75724 Paris Cedex 15, France

*To whom correspondence should be addressed

Received June 14, 1991. Accepted September 6, 1991.

The mouse heavy chain immunoglobulin promoter VH441 can lead in vitro to bidirectional transcription, due to a symmetrical organization of immunoglobulin heavy chain promoters with two TATA-like sequences bracketing the upstream promoter element ATGCAAAT (the so called octamer). We demonstrate here that divergent transcription also occurs in vivo in mature B cells from a myeloma which expresses the VH441 gene and even from the spleen of BALB/c mice. The level of VH441 divergent transcript increases in the spleen of BALB/c mice after immunisation by ß-(1,6)- galactan, showing that it is expressed in B cells which actively transcribe the VH441 gene. The divergent transcript has been characterized: its major transcription start site was mapped within 33 base pairs from the divergent TATA-like region, it is unspliced and not polyadenylated. In the light of these results, the functions of the divergent transcript and the bidirectional promoter are discussed.


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