Nucleic Acids Research, 1991, Vol. 19, No. 19 5351-5358
© 1991
MOLECULAR BIOLOGY |
Coregulation of the Kluyveromyces lactis lactose permease and ß-galactoidase genes is achieved by interaction of multiple LAC9 binding sites in a 2.6 kbp divergnent promoter
Institute of Microbiology, heinrich-Heine-Universität Düsseldorf 4000 düsseldrof 1, FRG
*To whom correspondence should be addressed
Received July 7, 1991. Accepted September 12, 1991.
The coregulated genes LAC4 and LAC12 encoding ß3-galactosidase and lactose permease, respectively, are responsible for the ability of the milk yeast Kluyveromyces lactis to utilise lactose. They are divergently transcribed and separated by an unusually large intergenic region of 2.6 kbp. Mapping of the upstream border of the ß3-galactosidase gene (LAC4) promoter by introduction of mutations at the chromosomal locus showed that LAC4 and LAC12 share the same upstream activation sites (UAS). The UASs represent binding sites for the trans-activator LAC9, a K.lactis homologue of GAL4, conforming to the consensus sequence 5'-CGG(N5)A/T(N5)CCG-3'. Two binding sites are located in front of each of the genes at almost symmetrical positions. ß3-galactosidase activity measurements as well as quantitation of LAC4 and LAC12 mRNA levels demonstrated that all four sites are required for full induction. LAC4 proximal anda LAC12 proximal sites cooperate in activating transcription of both genes. These sites are more than 1.7 kbp apart and the distal site is located more than 2.3 kbp upstream of the respective start of transcription. Thus, the distance between interacting sites is larger than in any of the well characterised yeast promoters. The contribution to gene activation differs for individual binding sites and correlates with the relative affinity of LAC9 for these sites in vitro suggesting that LAC9 binding is a rate limiting step for LAC promoter function.
+Present address: Max-Delbrück-Laboratorium, Carl-von-Linnè-Weg 10, 5000 Köln 30, FRG
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