Nucleic Acids Research, 1991, Vol. 19, No. 20 5763-5769
© 1991
MOLECULAR BIOLOGY |
The isolation and characterization of a novel cDNA demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells
Department of Microbiology and Molecular Genetics, School of Medicine, University of California Irvine, CA 92717 1Lineburger Cancer Research Center, University of North Carolina Chapel Hill, NC 27516, USA 2department of Cell Biology, School of Medicine, Vanderbilt University Nashville, TN 37232, USA
*To whom correspondence should be addressed
Received May 16, 1991. Accepted August 30, 1991.
Wilms‘ tumor, a pediatric nephroblastoma, has been associated with genetic alterations of the 11p13 and 11p15 regions. The introduction of a der(11) chromosome into the G401 Wilms‘ tumor cell line has been shown previously to revert the tumorigenic phenotype. A subtractive cDNA/RNA hybridization performed between the tumorigenic parent (G401) and a nontumorigenic microcell hybrid of G401 (110.1/G401.1) containing the der(11) chromosome resulted in the identification of a single novel cDNA clone, designated QM. The cDNA is 745 nucleotides in length and encodes a predicted hydrophilic 25 kd basic protein, primarily consisting of alpha helices. The QM transcript is expressed in a wide variety of embryonic and adult tissues and demonstrates a down regulation of expression in adult kidney and heart. QM is also a member of a multigene family members of which map to chromosomes 6 and 14. The QM mRNA level is modulated between the tumorigenic and nontumorigenic cell lines and therefore may be involved in the maintenance of the nontumorigenic phenotype.
+Present address: The Whitehead Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
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