Skip Navigation

This Article
Right arrow Print PDF (5760K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Yamamoto, K.
Right arrow Articles by Kyogoku, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, K.
Right arrow Articles by Kyogoku, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1991, Vol. 19, No. 22 6107-6112
© 1991

MOLECULAR BIOLOGY

Identification of transcriptional suppressor proteins that bind to the negative regulatory element of the human immunodeficiency virus type 1

Kazuo Yamamoto, Shigehisa Mori1, Takashi Okamoto1, Kunitada Shimotohno1 and Yoshimasa Kyogoku*

1National Cancer Center, Research Institute Tsukiji, Chuo-ku, Tokyo 104, Japan Institute for Protein Research, Osaka University Suita, Osaka 565, Japan

*To whom correspondence should be addressed

Received September 16, 1991. Accepted October 7, 1991.

Two different proteins which independently bound to neighboring sequences within the negative regulatory element (NRE) of human immunodeficiency virus type 1 (HIV-1) were detected in the nuclear extract of a virusinfected human T cell line. One of the factors bound to a novel dyad symmetrical sequence. This sequence is well conserved in various HIV-1 isolates and partial homology was found with the promoter region of the human retinoblastoma gene. Similar DNA binding activity was detected in a variety of virus-uninfected human T cell lines and HeLa cells by means of a gel mobility shift assay. The other factor bound to a putative AP-1 recognition sequence predicted for the HIV-1 NRE. However, this factor did not bind to a typical AP-1 site. The insertion of multiple copies of the binding site for the former or latter factor into a heterologous promoter reduced the promoter activity to one-tenth or one-third, espectively. Thus, each factor may function as a novel negative regulator of transcription.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Protein Eng Des SelHome page
M. Shimizu, H. Hiroaki, D. Kohda, T. Hosoya, Y. Akiyama-Oda, Y. Hotta, E. H. Morita, and K. Morikawa
NMR and ICP spectroscopic analysis of the DNA-binding domain of the Drosophila GCM protein reveals a novel Zn2+-binding motif
Protein Eng. Des. Sel., April 1, 2003; 16(4): 247 - 254.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. A. Pereira, K. Bentley, A. Peeters, M. J Churchill, and N. J. Deacon
SURVEY AND SUMMARY A compilation of cellular transcription factor interactions with the HIV-1 LTR promoter
Nucleic Acids Res., February 1, 2000; 28(3): 663 - 668.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.