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Nucleic Acids Research, 1991, Vol. 19, No. 22 6133-6138
© 1991

MOLECULAR BIOLOGY

Two independent promoters as well as 5'untranslated regions regulate Dd ras expression in Dictyostellium

Jean-François Louvion1, Jean-Charles Scholder, Stèphane Pinaud and Christophe D. Reymond*

Swiss Institute for Experimental Cancer Research ISREC, CH-1066 Epalinges 1Dèpartment de Biologie Cellulaire, Universitè de Genéve, Sciences III CH-1211 Genéve 4, Switzerland

*To whom correspondence should be addressed

Received September 9, 1991. Revised October 21, 1991. Accepted October 21, 1991.

The Dd ras gene produces three transcripts during Dictyostelium development. The largest transcript (L-) can be induced by external addition of cAMP even in cells prevented from aggregating, whereas shorter transcripts (S1- and S2-) expression requires cell aggregate formation. We show the presence of two independent promoters for L- and S-transcripts by deletion analysis of Dd ras fragments fused to CAT reporter genes reintroduced in Dictyostelium. A direct repeat upstream of S-transcript start sites which seems involved in S-promoter function, modulates also L-RNA accumulation. Furthermore removal of sequences between this repeat and the AUG protein start codon reduces the level of L-transcripts in aggregates. This study allowed to uncover the intricate pattern of sequences participating in the regulation of Dd ras expression.


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