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Nucleic Acids Research, 1991, Vol. 19, No. 22 6145-6153
© 1991

MOLECULAR BIOLOGY

Interplay of an original combination of factors: C/EBP, NFY, HNF3, and HNF1 in the rat aldolase B gene promoter

M. Raymondjean, A.L. Pichard, C. Gregori, F. Ginot and A. Kahn

ICGM, Laboratoire de Recherches en Gènètique et Pathologie Molèculaires, INSERM U 129, 24 rue du Faubourg Saint Jacques, 75014 Paris, France

Received September 5, 1991. Revised October 21, 1991. Accepted October 21, 1991.

The rat aldolase B 5' flanking region (nucleotides –194 to +41) contains sufficient information for liverspecific expression. A detailed investigation of factors binding to the rat aldolase B 5' flanking region has allowed us to identify three distinct factors that filled different sites of this region (A, B, C). The liver-enriched C/EBP or related factors bind to box C, as demonstrated by the specific interaction with bacterially expressed C/EBP protein. Box B bearing the CCAAT sequence binds the ubiquitous factor NFY. Surprisingly, Box A is able to bind two liver enriched factors, namely HNF1 and HNF3. However, in the context of the intact promoter, as shown by footprinting competition experiments, HNF3 binds solely to this sequence. HNF3, but not HNF1 is a transcriptional activator as demonstrated in the in vitro transcription assay.


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