Nucleic Acids Research, 1991, Vol. 19, No. 22 6155-6161
© 1991
MOLECULAR BIOLOGY |
High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110
MRC Virology Unit Church Street, Glasgow G11 5JR, UK
Received September 5, 1991. Revised October 15, 1991. Accepted October 15, 1991.
Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.
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