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Nucleic Acids Research, 1991, Vol. 19, No. 22 6277-6281
© 1991

MOLECULAR BIOLOGY

Leishmania tarentolae minicircles of different sequence classes encode single guide RNAs located in the variable region approximately 150 bp from the conserved region

Nancy R. Sturm+ and Larry Simpson*

Department of Biology and Molecular Biology Institute, University of California Los Angeles, CA 90024, USA

*To whom correspondence should be addressed

Received July 24, 1991. Revised October 15, 1991. Accepted October 15, 1991.

The complete sequences of three kinetoplast DNA minicircles (B4, D3 and D12) from Leishmania tarentolae are reported. All L. tarentolae minicircles encode single gRNAs localized within the variable region approximately 150 bp from the conserved region. The 5' termini and tentative 3' termini of the new gRNAs were determined and the gene sequences and flanking sequences of all minicircle gRNA genes compared for conserved motifs of possible transcriptional regulatory significance. All minicircle gRNAs possess 3' oligo-[U] tails of variable length similar to maxicircle gRNAs. A role for the D3 minicircle gRNA in the editing of the 5' pan-edited MURF4 mRNA was suggested by sequence analysis, and a role for the D12 minicircle gRNA in the editing of the COM mRNA and another minicircle gRNA (Lt154) in the editing of the pan-edited G6 mRNA have been previously reported. The cryptogene mRNAs edited by the B4 and Lt19 minicircle gRNAs are yet undetermined.

+Present address: Boyce Thompson Institute for Plant Research, Cornell University, Tower Rd, Ithaca, NY 14852, USA


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