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Nucleic Acids Research, 1991, Vol. 19, No. 23 6367-6372
© 1991

MOLECULAR BIOLOGY

More than half of yeast U1 snRNA is dispensabel for growth

Paul G. Siliciano1,2,*, Wendy J. Kivens2 and Christine Guthrie1

1Department of Biochemistry and Biophysics, University of California at San Francisco San Francisco, CA 94143 2Department of Biochemistry and Institute of Human Genetics, University of Minnesota Medical School 4-225 Millard Hall, 435 Delaware Street SE, Minneapolis, MN 55455, USA

*To whom correspondence should be addressed at Department of Biochemistry, University of Minnesota, 4-225 Millard Hall, 435 Delaware Street, SE Minneapolis, MN 55455, USA

Received October 2, 1991. Accepted October 21, 1991.

Yeast U1 snRNA (568 nucleotides) is 3.5-fold larger than its mammalian counterpart (164 nucleotides) and contains apparent sequence homology only at the 5' and 3' ends. We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. The minimal essential sequences of yeast U1 can be drawn in a secondary structure which resembles metazoan U1 in four of seven structural domains.


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