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Nucleic Acids Research, 1991, Vol. 19, No. 23 6399-6404
© 1991

MOLECULAR BIOLOGY

Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein

Andrew Collick, Michael G. Dunn and Alec J. Jeffreys

Department of Genetics, University of Leicester University Road, Leicester LE1 7RH, UK

Received September 19, 1991. Accepted November 11, 1991.

Msbp-1 is a minisatellite-specific DNA-binding protein. Using synthetic binding substrates, we now show that Msbp-1 binds not to double-stranded DNA, but exclusively to single-stranded DNA. Binding is specific to the guanine-rich strand of the minisatellite duplex, interactions with the cytosine-rich strand being undetectable by southwestern analysis. Furthermore, the binding site required for successful DNA-protein interactions appears to be two or more minisatellite repeat units. We have also isolated, by whole-genome PCR and cloning, one Msbp-1 binding site from the human genome. Again, the binding strand of this molecule contains a repetitive G-rich structure equivalent to that of a small minisatellite. These observations are discussed with respect to other single-stranded DNA-binding proteins known to play a role in recombination processes.


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