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Nucleic Acids Research, 1991, Vol. 19, No. 23 6481-6483
© 1991

MOLECULAR BIOLOGY

A trace display and editing program for data from fluorescence based sequencing machines

Timothy Gleeson and LaDeana Hillier1,*

MRC Laboratory of Molecular Biology Hills Road, Cambridge CB2 2QH, UK 1Washington University School of Medicine, Department of Genetics Box 8232, 4566 Scott Avenue, St Louis, MO 63110, USA

*To whom correspondence should be addressed

Received August 26, 1991. Accepted November 30, 1991.

‘Ted’ (Trace editor) is a graphical editor for sequence and trace data from automated fluorescence sequencing machines. It provides facilities for viewing sequence and trace data (in top or bottom strand orientation), for editing the base sequence, for automated or manual trimming of the head (vector) and tail (uncertain data) from the sequence, for vertical and horizontal trace scaling, for keeping a history of sequence editing, and for output of the edited sequence. Ted has been used extensively in the C.elegans genome sequencing project, both as a standalone program and integrated into the Staden sequence assembly package, and has greatly aided in the efficiency and accuracy of sequence editing. It runs in the X windows environment on Sun workstations and is available from the authors. Ted currently supports sequence and trace data from the ABI 373A and Pharmacia A.L.F. sequencers.


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