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Nucleic Acids Research, 1991, Vol. 19, No. 25 7061-7065
© 1991

Foreword

Enhancer effect of bovine papillomavirus E2 protien in replication of polyomavirus DNA

Mats Nilsson, Maud Forsberg1, Zhiyong You, Gunnar Westin1 and Göran Magnuson*

Departments of Medical Virology Box 584, S-751 23 Uppsala, Sweden 1Medical Genetics, Uppsala University, Biomedical Center Box 584, S-751 23 Uppsala, Sweden

*To whom correspondence should be addressed

In polyomavirus, both transcription from the early promoter and viral DMA replication initiated at the origin of DNA replication is controlled by binding of proteins to the enhancer region. We have developed a simple model system to study the role of an enhancer binding factor in the initiation of polyomavirus DNA replication.

A reporter plasmid was constructed which has the enhancer region replaced by two binding sites for a transcription factor, the E2 protein encoded by bovine papillomavirus type 1. Co-transfectlon of COP 5 cells which express polyomavirus large T-antigen, with the reporter plasmid and an E2 expression plasmid, resulted in E2-dependent stimulation of replication of the reporter plasmid. The activity of several E2 mutants was also analysed. Mutant proteins with decreased activity in transcriptional trans-activation, resulting from impaired DNA binding or other defects, were found to have quantitatively similar reductions of activity in viral DNA replication. The results suggest that the E2 protein activates transcription and polyomavirus DNA replication by similar mechanism(s).


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