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Nucleic Acids Research, 1991, Vol. 19, No. 25 7171-7175
© 1991

Foreword

Mouse embryonic stem cells exhibit high levels of extrachromosomal homologous recombination in a chloramphenicol acetyltransferase assay system

Maria Jasin and Feng Liang

Department of Cell Biology and Genetics and Program of Molecular Biology, Sloan-Kettering Institute and Cornell University Graduate School of Medical Sciences 1275 York Avenue, New York,NY 10021, USA

Mouse embryonic stem (ES) cells were compared to COS1 and CV1 cells for their ability to perform extrachromosomal homologous recombination. RSVCAT plasmid substrates consisting of overlapping chloramphenlcol acetyltransferase (CAT) gene fragments were transiently transfected into cells and extracts were assayed for CAT activity. Approximately 10% activity, relative to transfectlon with a complete CAT gene, was recovered for the recombination substrates In each of the cell lines tested. ES cells, therefore, as other cell lines, are capable of high levelsof extrachromosomal recombination.


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