Nucleic Acids Research, 1991, Vol. 19, No. 3 435-441
© 1991
MOLECULAR BIOLOGY |
The different positioning of the proximal sequence element in the Xenopus RNA polymerase II and III snRNA promoters is a key determinant which confers RNA polymerase III specificity
Laboratoire de Biochimie, Institut de Biologie Moleculaire et Cetlulaire du CNRS 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
*To whom correspondence should be addressed
Received November 27, 1990. Revised January 3, 1991. Accepted January 3, 1991.
We and others have previously described the TATA motif as a major determinant for Pol III specificity of the U6 promoter. Surprisingly, however, the data documented here show that the sole introduction of a TATA sequence into a U1 Pol II snRNA gene is not sufficient to confer Pol III transcription. Rather, this promoter element can mediate optimal Pol III transcription only if the PSE, the second promoter element, is shifted 4 bp upstream of the position it occupies in Pol II snRNA genes. As a result, the PSE-TATA-start site spacing introduced into the U1 Pol II gene is identical to that of the U6 gene and is strictly required to produce properly initiated Pol III transcripts. Thus, Pol II and Pol III PSEs, although similar in sequence, are not positionally equivalent. Competitive experiments raise the possibility that vertebrate U6 genes contain other, as yet unidentified, promoter elements.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. B. Patel and M. Bellini The assembly of a spliceosomal small nuclear ribonucleoprotein particle Nucleic Acids Res., November 1, 2008; 36(20): 6482 - 6493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Hernandez Jr, F. Valafar, and W. E. Stumph Insect small nuclear RNA gene promoters evolve rapidly yet retain conserved features involved in determining promoter activity and RNA polymerase specificity Nucleic Acids Res., January 12, 2007; 35(1): 21 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hinas, P. Larsson, L. Avesson, L. A. Kirsebom, A. Virtanen, and F. Soderbom Identification of the Major Spliceosomal RNAs in Dictyostelium discoideum Reveals Developmentally Regulated U2 Variants and Polyadenylated snRNAs Eukaryot. Cell, June 1, 2006; 5(6): 924 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Myslinski, J.-C. Ame, A. Krol, and P. Carbon An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene Nucleic Acids Res., June 15, 2001; 29(12): 2502 - 2509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Paule and R. J. White SURVEY AND SUMMARY Transcription by RNA polymerases I and III Nucleic Acids Res., March 15, 2000; 28(6): 1283 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Roberts, T Colbert, and S Hahn TFIIIC determines RNA polymerase III specificity at the TATA-containing yeast U6 promoter. Genes & Dev., April 1, 1995; 9(7): 832 - 842. [Abstract] [PDF]
|
|
|
|
|
|
C L Sadowski, R W Henry, S M Lobo, and N Hernandez Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE. Genes & Dev., August 1, 1993; 7(8): 1535 - 1548. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Dahlberg and E Lund How does III x II make U6? Science, December 6, 1991; 254(5037): 1462 - 1463. [PDF]
|
|
|
|
|
|
S M Lobo, J Lister, M L Sullivan, and N Hernandez The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro. Genes & Dev., August 1, 1991; 5(8): 1477 - 1489. [Abstract] [PDF]
|
|