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Nucleic Acids Research, 1991, Vol. 19, No. 3 443-448
© 1991

MOLECULAR BIOLOGY

A bacterial model system for chromosomal targeting

L.-C. Huang, E.A. Wood and Michael MCox*

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin– Madison Madison, Wl 53706, USA

*To whom correspondence should be addressed

Received November 26, 1990. Accepted December 31, 1990.

A system that permits efficient site-specific chromosomal targeting of foreign DNA on the Escherichia coli chromosome has been developed, using the FLP site-specific recombination system derived from the yeast 2µ plasmld. The system demonstrates the feasibility of using site-specific recombination for this purpose, and provides a means to gather information on parameters that may affect chromosomal targeting to guide efforts to establish similar systems in higher eukaryotes. In this model system, the efficiency of integration of foreign DNA is affected by the location of the target site in the chromosome, and the structure of the recombination sites.


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