Nucleic Acids Research, 1991, Vol. 19, No. 3 461-468
© 1991
MOLECULAR BIOLOGY |
Cloning and sequence determination of the Schizosaccharomyces pombe rpb1 gene encoding the largest subunit of RNA polymerase II
Department of Molecular Genetics, National Institute of Genetics Mishima, Shizuoka 411, Japan
*To whom correspondence should be addressed
Received November 22, 1990. Revised December 28, 1990. Accepted December 28, 1990.
The gene, rpbl, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB, of Saccharomyces cerevlslae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six Introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptlde found in all the eukaryotic RNA polymerase II largest subunits so far examined.
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