Nucleic Acids Research, 1991, Vol. 19, No. 3 485-491
© 1991
MOLECULAR BIOLOGY |
The promoter of the chicken 
2(VI) collagen gene has features characteristic of house-keeping genes and of proto-oncogenes
Laboratorium f
r Biochemie I, Eidgenössische Technische Hochschule Zrich ETH-Zentrum, CH-8092 Zrich, Switzerland
*To whom correspondence should be addressed
Received November 19, 1990. Revised January 7, 1991. Accepted January 7, 1991.
The promoter of the chicken 
2(VI) collagen gene reveals several Interesting features characteristic of house-keeping genes and growth control related genes. It does not possess a typical TATAA or CAAT box, but it contains several potential binding sites for transcription factors Sp1 and ETF. The 5' flanking region of the gene forms a typical ‘CpG island’ where the dinucleotide sequence CpG occurs with high frequency relative to the bulk genome. Consistent with the lack of a TATAA element, the gene contains multiple transcription initiation sites distributed over 75 bp of genomlc DNA. A short DNA fragment (207 bp) encompassing all the transcription initiation sites and the entire CpG island shows strong promoter activity when linked to a heterologous reporter gene. The upstream region of the promoter harbours a long homopurine/homopyrimidine element (403 bp) which is sensitive to endonuclease S1. This element might have the ability to adopt an Intramolecular hairpin triplex structure and could play a role in the organization of the chromatin at the 2(VI) collagen locus. Our results demonstrate that the structure of the 2(VI) collagen promoter is completely different from that of any other collagen promoter characterized so far.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. A. McCormick, V. Lyons, M. D. Jacobson, J. Noble, J. Diorio, M. Nyirenda, S. Weaver, W. Ester, J. L. W. Yau, M. J. Meaney, et al. 5'-Heterogeneity of Glucocorticoid Receptor Messenger RNA Is Tissue Specific: Differential Regulation of Variant Transcripts by Early-Life Events Mol. Endocrinol., April 1, 2000; 14(4): 506 - 517. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Reinhard, J. Zumbrunn, D. Jaquemar, M. Kuhn, U. Walter, and B. Trueb An alpha -Actinin Binding Site of Zyxin Is Essential for Subcellular Zyxin Localization and alpha -Actinin Recruitment J. Biol. Chem., May 7, 1999; 274(19): 13410 - 13418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Xu and A. G. Goodridge Characterization of a Polypyrimidine/Polypurine Tract in the Promoter of the Gene for Chicken Malic Enzyme J. Biol. Chem., July 5, 1996; 271(27): 16008 - 16019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Piccolo, P. Bonaldo, P. Vitale, D. Volpin, and G. M. Bressan Transcriptional Activation of the alpha1(VI) Collagen Gene during Myoblast Differentiation Is Mediated by Multiple GA Boxes J. Biol. Chem., August 18, 1995; 270(33): 19583 - 19590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Quarto, B. Dozin, P. Bonaldo, R. Cancedda, and A. Colombatti Type VI collagen expression is upregulated in the early events of chondrocyte differentiation Development, January 1, 1993; 117(1): 245 - 251. [Abstract] [PDF]
|
|