Nucleic Acids Research, 1991, Vol. 19, No. 3 591-598
© 1991
MOLECULAR BIOLOGY |
Differential response of the human 6–16 and 9–27 genes to 
and 
interferons
Imperial Cancer Research Fund PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK
*To whom correspondence should be addressed
Received October 1, 1991. Revised January 7, 1990. Accepted January 7, 1990.
9–27 mRNA is expressed to a high level in response to both 
and 
interferons. In contrast, 6–16 mRNA Is expressed well in response to but very poorly in response to interferon in human cells. The factors governing these different levels of expression were investigated. For both genes the major effect of both interferons is on transcription. A transcriptional bias in the 6–16 promoter/enhancer accounts in large part for the differential response of 6–16 to the two interferons. No single DNA element appears responsible; the smaller the 5' region analysed the lower the absolute activity and the smaller the differential response to and interferons observed. Both the 6–16 and 9–27 mRNAs are very stable and no effect of the interferons on stability was detected. Nor was any direct evidence obtained for preferential processing of the 9–27 mRNA. Nevertheless, differentials between the transcription and accumulation of mature mRNAs, particularly for 6–16 mRNA in response to interferon, suggest that post-transcriptional controls) must additionally operate. The 9–27 5' promoter/enhancer Is much less active than that from 6–16 when placed 5' of a marker gene, despite the similar response of the two genes to a interferon.
+Present addresses: Editorial Office, Cell, 50 Church Street, Cambridge, MA 02138, USA
Wellcome Biotech, Langley Court, Beckenham BR3 3BS, Kent
øBiochemistry, University of Oxford, South Parks Road, Oxford 0X1 3QU, UK
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