Nucleic Acids Research, 1991, Vol. 19, No. 3 599-603
© 1991
MOLECULAR BIOLOGY |
Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium
Institute for Microbial and Biochemical Technology, Forest Products Laboratory One Gifford Pinchot Drive, Madison, Wl 53705, USA
*To whom correspondence should be addressed
Received September 25, 1990. Revised January 7, 1991. Accepted January 7, 1991.
Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmld insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresls (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.
+Present address: University of Minnesota Medical School, Minneapolis, MN, USA
![]()
CiteULike
Connotea
Del.icio.us What's this?
This article has been cited by other articles:
![]() |
L. M. Larraya, G. Pérez, E. Ritter, A. G. Pisabarro, and L. Ramírez Genetic Linkage Map of the Edible Basidiomycete Pleurotus ostreatus Appl. Envir. Microbiol., December 1, 2000; 66(12): 5290 - 5300. [Abstract] [Full Text] |
||||
![]() |
P. Stewart, J. Gaskell, and D. Cullen A Homokaryotic Derivative of a Phanerochaete chrysosporium Strain and Its Use in Genomic Analysis of Repetitive Elements Appl. Envir. Microbiol., April 1, 2000; 66(4): 1629 - 1633. [Abstract] [Full Text] |
||||
![]() |
P. Stewart and D. Cullen Organization and Differential Regulation of a Cluster of Lignin Peroxidase Genes of Phanerochaete chrysosporium J. Bacteriol., June 1, 1999; 181(11): 3427 - 3432. [Abstract] [Full Text] |
||||
![]() |
B. Li and V. Renganathan Gene Cloning and Characterization of a Novel Cellulose-Binding beta -Glucosidase from Phanerochaete chrysosporium Appl. Envir. Microbiol., July 1, 1998; 64(7): 2748 - 2754. [Abstract] [Full Text] |
||||

