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Nucleic Acids Research, 1991, Vol. 19, No. 3 599-603
© 1991


MOLECULAR BIOLOGY

Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium

Jill Gaskell, Eric Dieperink+ and Daniel Cullen*

Institute for Microbial and Biochemical Technology, Forest Products Laboratory One Gifford Pinchot Drive, Madison, Wl 53705, USA

*To whom correspondence should be addressed

Received September 25, 1990. Revised January 7, 1991. Accepted January 7, 1991.

Three lignin peroxidase (LiP) genes from the basidiomycete Phanerochaete chrysosporium were cloned on a single 30 kb cosmld insert. One gene, GLG5, is the genomic equivalent of a previously reported cDNA clone, CLG5. The other two LiP genes are transcriptionally convergent and map to a position approximately 15 kb downstream of GLG5. The translational stop codons of these genes are separated by 1.3 kb. Analysis of homokaryons established allelic relationships to previously described LiP clones. Using clamped homogeneous electrical field electrophoresls (CHEF), seven chromosomal bands were resolved from P. chrysosporium genomic DNA. On CHEF gel Southern blots, the LiP gene family was localized to a single, dimorphic chromosome.


+Present address: University of Minnesota Medical School, Minneapolis, MN, USA


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