Skip Navigation

This Article
Right arrow Print PDF (5058K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Partono, S.
Right arrow Articles by Lewin, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Partono, S.
Right arrow Articles by Lewin, A. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1991, Vol. 19, No. 3 605-609
© 1991

MOLECULAR BIOLOGY

The rate and specificity of a group I ribozyme are inversely affected by choice of monovalent salt

Steve Partono and Alfred S. Lewin*

Department of Immunology and Medical Microbiology, University of Florida College of Medicine Box J266, Gainesville, FL 32610, USA

*To whom correspondence should be addressed

Received September 24, 1990. Revised December 13, 1990. Accepted December 13, 1990.

The fifth intron of the COB gene of yeast mitochondria splices autocatalytically. The rate of splicing is increased by high concentrations of monovalent salts, but the choice of both cation and anion is significant: The smaller the cation In solution, the faster the reaction (the rate in K+ > NH4+ ≥ Na+ > Li+). Chloride, bromide, iodide and acetate salts enhance autocatalytic processing, but sulfate salts do not and fluoride salts are inhibitory. The choice of monovalent salt affects the KM of the intron for guanosine nucleotide, implying an alteration in the affinity of the RNA for that substrate. Under optimal conditions (1M KCI, 50 mM MgCl2) the catalytic efficiency of this intron exceeds that reported for the ribosomal intron from Tetrahymena, but several side reactions occur, including guanosine-addition within the downstream exon. The site of addition resembles the 5' splice junction, but selection of this site does not involve the internal guide sequence of the Intron.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.