Nucleic Acids Research, 1991, Vol. 19, No. 4 747-750
© 1991
CHEMISTRY |
Modified deoxyoligonucleotides stable to exonuclease degradation in serum
Gilead Sciences 346 Lakeside Drive, Foster City, CA 94404, USA
* To whom correspondence should be addressed
Received November 30, 1990. Revised January 8, 1991. Accepted January 8, 1991.
Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoilgonucleotides are protected from exonuclease degradation In sera and cell media by simple terminal modifications that maintain high binding affinity for the complementary DNA sequence.
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