Nucleic Acids Research, 1991, Vol. 19, No. 4 789-794
© 1991
MOLECULAR BIOLOGY |
A sequence motif found in a Drosophila heterochromatin protein is conserved in animals and plants
Department of Molecular Embryology, Institute of Animal Physiology and Genetics Research, Cambridge Research Station Babraham, Cambridge CB2 4AT, UK 1Zentrum für Molekulare Biologic, Universität Heidelberg D-6900 Heidelberg 1, FRG 2Molecular Biology and Biochemistry Department, Wesleyan University Middletown, CT 06457 - 6032, USA
Received November 20, 1990. Revised January 21, 1991. Accepted January 21, 1991.
Modifiers of position-effect-variegation in Drosophila encode proteins that are thought to modify chromatin, rendering it heritably changed in its expressibiiity. In an attempt to identify similar modifier genes in other species we have utilized a known sequence homology, termed chromo box, between a suppressor of positioneffect- variegation, Heterochromatin protein 1 (HP1), and a repressor of homeotic genes, Polycomb (PC). A PCR generated probe encompassing the HP1 chrorno box was used to clone full-length murine cDNAs that contain conserved chrorno box motifs. Sequence comparisons, in situ hybridization experiments, and RNA Northern blot analysis suggest that the murine and human sequences presented in this report are homoiogues of the Drosophila HP1 gene. Chromo box sequences can also be detected in other animal species, and in plants, predicting a strongly conserved structural role for the peptide encoded by this sequence. We propose that epigenetic (yet heritable) changes in gene expressibility, characteristic of chromosomal imprinting phenomena, can largely be explained by the action of such modifier genes. The evolutionary conservation of the chromo box motif now enables the isolation and study of putative modifier genes in those animal and plant species where chromosomal imprinting has been described.
* Present address: Institut du Cancer de Montréal, 1560 rue Sherbrooke est, Montréal, Québec. Canada H2L 4M1
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