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Nucleic Acids Research, 1991, Vol. 19, No. 4 871-875
© 1991

ENZYMOLOGY

Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer

Karl D. Bishop, Philip N. Borer*, Yao-Qi Huang1 and Michael j. Lane1,*

Department of Chemistry and NMR and Data Processing Laboratory, Syracuse University Syracuse, NY 13244 - 4100 1Division of Hematology/Oncology, Departments of Medicine and Microbiology, State University of New York - Health Science Center at Syracuse Syracuse, NY 13210, USA

* To whom correspondence should be addressed

Received October 15, 1990. Revised January 14, 1991. Accepted January 14, 1991.

DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5'-to-3' direction from the drug binding site. An inequivalent response of the two strands to a ligandinduced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.


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