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Nucleic Acids Research, 1991, Vol. 19, No. 5 1093-1098
© 1991

MOLECULAR BIOLOGY

The U1 snRNA gene repeat from the sea urchin (Strongylocentrotus purpuratus): the 70 kilobase tandem repeat ends directly 3' to a U1 gene

Jin-Chen Yu+, Brian Wendelburg, Sameer Sakallah and William F. Marzluff*

Department of Chemistry and Institute of Molecular Biophysics, Florida State University Tallahassee, FL 32306, USA

* To whom correspondence should be addressed

Received November 20, 1990. Revised January 29, 1991. Accepted January 29, 1991.

Lambda phage clones containing multiple copies of the 1.1 kb tandemly repeated unit of the sea urchin (S. purpuratus) U1 RNA genes were isoiated from a gene library. The 1.1kb repeat unit encodes a single copy of the predominant U1 RNA expressed in oocytes and embryos prior to the blastula stage. The tandem repeat unit is about 80 kb in size and is probabiy present one time per haplold genome as judged by pulsed-field eiectrophoresis of sperm DNA digested with restriction enzymes which do not cut in the repeat unit. Two of the phage contained DNA flanking the repeat unit as well as several repeat units. The tandem repeat unit ends Just 3' to the U1 coding region. There is oniy iimited homology in the 5' flanking region with U1 snRNA genes from the sea urchin L. variegatus.

+Present address: Tumor Virology Section, Building 37, National Institutes of Health, Bethesda, MD 20205, USA


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