Nucleic Acids Research, 1991, Vol. 19, No. 6 1197-1201
© 1991
MOLECULAR BIOLOGY |
Site-specific creation of uridine from cytidine in apolipoprotein B mRNA editing
Division of Molecular Medicine, MRC Clinical Research Centre Watford Road, Harrow, Middlesex HA1 3UJ, UK
* To whom correspondence should be addressed
Received January 22, 1991. Accepted February 21, 1991.
Human apolipoprotein (apo) B mRNA is edited in a tissue specific reaction, to convert glutamine codon 2153 (CAA) to a stop translation codon. The RNA editing product templates and hybridises as uridine, but the chemical nature of this reaction and the physical identity of the product are unknown. After editing in vitro of [32P] labelled RNA, we are able to demonstrate the production of uridine from cytidine; [
32P] cytidine triphosphate incorporated into RNA gave rise to [32P] uridine monophosphate after editing in vitro, hydrolysis with nuclease P1 and thin layer chromatography using two separation systems. By cleaving the RNA into ribonuclease T1 fragments, we show that uridine is produced only at the authentic editing site and is produced in quantities that parallel an independent primer extension assay for editing. We conclude that apo B mRNA editing specifically creates a uridine from a cytidine. These observations are inconsistent with the incorporation of a uridine nucleotide by any polymerase, which would replace the -phosphate and so rule out a model of endonucleolytic excision and repair as the mechanism for the production of uridine. Although transaminatJon and transglycosylation remain to be formally excluded as reaction mechanisms our results argue strongly in favour of the apo B mRNA editing enzyme as a site-specific cytidine deaminase.
1Present address: Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Zoltowska, E. Ziv, E. Delvin, M. Lambert, E. Seidman, and E. Levy Both Insulin Resistance and Diabetes in Psammomys obesus Upregulate the Hepatic Machinery Involved in Intracellular VLDL Assembly Arterioscler. Thromb. Vasc. Biol., January 1, 2004; 24(1): 118 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hersberger, S. Patarroyo-White, K. S. Arnold, and T. L. Innerarity Phylogenetic Analysis of the Apolipoprotein B mRNA-editing Region. EVIDENCE FOR A SECONDARY STRUCTURE BETWEEN THE MOORING SEQUENCE AND THE 3' EFFICIENCY ELEMENT J. Biol. Chem., December 3, 1999; 274(49): 34590 - 34597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Greeve, D. Axelos, S. Welker, M. Schipper, and H. Greten Distinct Promoters Induce APOBEC-1 Expression in Rat Liver and Intestine Arterioscler. Thromb. Vasc. Biol., July 1, 1998; 18(7): 1079 - 1092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hersberger and T. L. Innerarity Two Efficiency Elements Flanking the Editing Site of Cytidine 6666 in the Apolipoprotein B mRNA Support Mooring-dependent Editing J. Biol. Chem., April 17, 1998; 273(16): 9435 - 9442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu and W. Schuster Evidence for a Site-specific Cytidine Deamination Reaction Involved in C to U RNA Editing of Plant Mitochondria J. Biol. Chem., August 4, 1995; 270(31): 18227 - 18233. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B Teng, C. Burant, and N. Davidson Molecular cloning of an apolipoprotein B messenger RNA editing protein Science, June 18, 1993; 260(5115): 1816 - 1819. [Abstract] [PDF]
|
|
|
|
|
|
H. Lellek, R. Kirsten, I. Diehl, F. Apostel, F. Buck, and J. Greeve Purification and Molecular Cloning of a Novel Essential Component of the Apolipoprotein B mRNA Editing Enzyme-Complex J. Biol. Chem., June 23, 2000; 275(26): 19848 - 19856. [Abstract] [Full Text] [PDF]
|
|