Nucleic Acids Research, 1991, Vol. 19, No. 6 1213-1217
© 1991
MOLECULAR BIOLOGY |
CAAT/enhancer binding protein is able to bind to ATF/CRE elements
Molecular Endocrinology Laboratory, Imperial Cancer Research Fund Lincoln's Inn Fields, London WC24 3PX, UK
* To whom correspondence should be addressed
Received January 4, 1991. Revised February 20, 1991. Accepted February 20, 1991.
The transcription factor C/EBP is known to be able to bind to two different classes of sites, a CAAT-box and one present in a number of viral enhancers. In this paper we show using band-shift assays, methylation interference and footprintlng that C/EBP is also able to bind with high affinity to ATF/CRE sites. Competition with mutant ATF sites and methylation interference indicate that C/EBP may be able to bind to the ATF/CRE sites by virtue of their homotogy to the enhancer core elements. Furthermore, we show that C/EBP is able to direct transcription from this site in transient transfection experiments and that mutations in the ATF binding site that impair DNA binding also effect the ability of C/EBP to stimulate transcription.
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