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Nucleic Acids Research, 1991, Vol. 19, No. 6 1219-1226
© 1991

MOLECULAR BIOLOGY

The promoter and enhancer of the inactive chicken ß-globin gene contains precisely positioned nucleosomes

Robin Buckle, Michael Balmer, Ali Yenidunya and James Allan*

Department of Biophysics, Cell and Molecular Biology, Division of Biomolecular Sciences, King's College 26 – 29 Drury Lane, London WC2B 5RL, UK

* To whom correspondence should be addressed

Received December 13, 1990. Revised February 15, 1991. Accepted February 15, 1991.

Core histone octamers reconstituted in vitro onto DNA fragments containing the chicken ß-globin gene promoter are precisely positioned with respect to the underlying DNA sequence [1]. Here we show that this is also true of the chicken ß-globin gene enhancer. These nucleosome binding sites are also employed within transfected COS cell nuclei, where the chicken ß-globin gene is transcriptionally inactive. Similar results were found in vivo, where positioned nucleosomes were detected over the inactive ß-globin promoter in chicken brain cells and 5-day red blood cells, and over the inactive ß-globin enhancer in brain cells. In contrast, the promoter and enhancer regions were found to be nucleosome-free in 15-day erythrocytes where the ß-globln gene Is active. We argue that these results suggest a role for positioned nucleosomes in the regulation of the transcription of the chicken ß-globin gene.


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