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Nucleic Acids Research, 1991, Vol. 19, No. 6 1227-1234
© 1991

MOLECULAR BIOLOGY

Two types of antiprogestins identified by their differential action in transcriptionally active extracts from T47D cells

Ludger Klein-Hitpass*, Andrew C.B. Cato1, David Henderson2 and Gerhart U. Ryffel

Institut für Zellbiologie, Tumorforschung, Universitätsklinikum Essen Hufelandstrasse 55, D-4300 Essen 1 1Kernforschungszentrum Karlsruhe, Institut fü Genetik und Toxikologie Postfach 3640, D-7500 Karlsruhe 2Research Laboratories of Schering AG, Department of Experimental Andrology and Oncology Müllerstrasse 170-178, D-1000 Berlin 65, FRG

* To whom correspondence should be addressed

Received December 31, 1990. Revised February 27, 1991. Accepted February 27, 1991.

Transcriptionally active nuclear extracts from human breast carcinoma cells (T47D) were used to compare the action of progestins and several antiprogestins of the 11ß-aryl substituted steroid series on the DNA- binding properties and the trans-activating potential of progesterone receptor (PR) In vitro. Using the gel-shift assay we identified a novel type of antiprogestin (ZK98299, type I), which in contrast to type II antiprogestins, including RU486, does not Induce binding of PR to progesterone response elements (PRES). In competition experiments excess of type I antiprogestin inhibits induction of DNA binding of PR by progestins and type II antiprogestins suggesting that its binding to PR interferes with the formation of stable receptor dimers. Moreover, we demonstrate that the antagonistic action of ZK98299 can be fully mimicked In vitro by using cell-free nuclear extracts from T47D cells and a ‘simple’ test promoter. In contrast, type II antiprogestins known to induce certain promoters In vivo exert strong agonistic effects on in vitro transcription of the test template used.


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