Nucleic Acids Research, 1991, Vol. 19, No. 6 1251-1258
© 1991
MOLECULAR BIOLOGY |
Transcriptional interference between the EBV transcription factors EB1 and R: both DNA-binding and activation domains of EB1 are required
ENS-CNRS UMR49, Ecole Normale Supéheure de Lyon 46 Allée d'ltalie, 69364 Lyon Cedex 07 1 IGR 39 rue C.Desmoulin, 94800 Villejuif 2Villejuif Department, Hopital Trousseau Avenue A.Netter, 75012 Paris, France
* To whom correspondence should be addressed
Received December 18, 1990. Revised February 15, 1991. Accepted February 15, 1991.
The switch from latency to a productive infection in EBV-infected B cells is linked to the expression of two viral sequence-specific DNA-binding transcription factors called EB1 and A. EB1 shares sequence homologles with the bZIP family of proteins In the basic region required for specific DNA Interaction. Here, we provide evidence that EB1 and R can synergistically activate specific transcription, and that overexpressed, unbound EB1, represses the R-lnduced transcription (‘squelching’). In order to identify the EB1 domains Involved in transcriptional activation, transcriptional synergy and transcriptional repression, we performed extensive mutagenesis of the EB1 protein. Results show that five segments (region 1 to region 5), localized at the N-terminus of EB1 exhibit characteristics of activating domains, since they are required for full transcriptional activity, without obvious role in DNA- binding, or the nuclear localization. Two domains rich in basic amino-acids are required for the nuclear localization of EB1. One domain is within the basic region B, also necessary for specific and stable interaction between EB1 and its cognate DNA sequences. It is also shown that the ‘activation’ domain, and more surprisingly the DNA-binding domain of EB1, may interact with a factor(s), essential for R-induced activation, and probably required for synergy between EB1 and R.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Li, L. Qian, C. Chen, M. Shi, M. Yu, M. Hu, L. Song, B. Shen, and N. Guo Down-Regulation of MHC Class II Expression through Inhibition of CIITA Transcription by Lytic Transactivator Zta during Epstein-Barr Virus Reactivation J. Immunol., February 15, 2009; 182(4): 1799 - 1809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Jones, S. Dickerson, P. M. Bhende, H.-J. Delecluse, and S. C. Kenney Epstein-Barr Virus Lytic Infection Induces Retinoic Acid-responsive Genes through Induction of a Retinol-metabolizing Enzyme, DHRS9 J. Biol. Chem., March 16, 2007; 282(11): 8317 - 8324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Adamson, N. Wright, and D. R. LaJeunesse Modeling Early Epstein-Barr Virus Infection in Drosophila melanogaster: The BZLF1 Protein Genetics, November 1, 2005; 171(3): 1125 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Izumiya, T. J. Ellison, E. T. H. Yeh, J. U. Jung, P. A. Luciw, and H.-J. Kung Kaposi's Sarcoma-Associated Herpesvirus K-bZIP Represses Gene Transcription via SUMO Modification J. Virol., August 1, 2005; 79(15): 9912 - 9925. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Adamson Epstein-Barr Virus BZLF1 Protein Binds to Mitotic Chromosomes J. Virol., June 15, 2005; 79(12): 7899 - 7904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. El-Guindy and G. Miller Phosphorylation of Epstein-Barr Virus ZEBRA Protein at Its Casein Kinase 2 Sites Mediates Its Ability To Repress Activation of a Viral Lytic Cycle Late Gene by Rta J. Virol., July 15, 2004; 78(14): 7634 - 7644. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mahot, A. Sergeant, E. Drouet, and H. Gruffat A novel function for the Epstein-Barr virus transcription factor EB1/Zta: induction of transcription of the hIL-10 gene J. Gen. Virol., April 1, 2003; 84(4): 965 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. El-Guindy, L. Heston, Y. Endo, M.-S. Cho, and G. Miller Disruption of Epstein-Barr Virus Latency in the Absence of Phosphorylation of ZEBRA by Protein Kinase C J. Virol., October 11, 2002; 76(22): 11199 - 11208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Adamson and S. Kenney Epstein-Barr Virus Immediate-Early Protein BZLF1 Is SUMO-1 Modified and Disrupts Promyelocytic Leukemia Bodies J. Virol., March 1, 2001; 75(5): 2388 - 2399. [Abstract] [Full Text]
|
|
|
|
 |
|
 L.-K. Chang and S.-T. Liu Activation of the BRLF1 promoter and lytic cycle of Epstein-Barr virus by histone acetylation Nucleic Acids Res., October 15, 2000; 28(20): 3918 - 3925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Aho, M. Buisson, T. Pajunen, Y. W. Ryoo, J.-F. Giot, H. Gruffat, A. Sergeant, and J. Uitto Ubinuclein, a Novel Nuclear Protein Interacting with Cellular and Viral Transcription Factors J. Cell Biol., March 20, 2000; 148(6): 1165 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Adamson, D. Darr, E. Holley-Guthrie, R. A. Johnson, A. Mauser, J. Swenson, and S. Kenney Epstein-Barr Virus Immediate-Early Proteins BZLF1 and BRLF1 Activate the ATF2 Transcription Factor by Increasing the Levels of Phosphorylated p38 and c-Jun N-Terminal Kinases J. Virol., February 1, 2000; 74(3): 1224 - 1233. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ragoczy and G. Miller Role of the Epstein-Barr Virus Rta Protein in Activation of Distinct Classes of Viral Lytic Cycle Genes J. Virol., December 1, 1999; 73(12): 9858 - 9866. [Abstract] [Full Text]
|
|
|
|
|
|
A. Rodriguez, M. Armstrong, D. Dwyer, and E. Flemington Genetic Dissection of Cell Growth Arrest Functions Mediated by the Epstein-Barr Virus Lytic Gene Product, Zta J. Virol., November 1, 1999; 73(11): 9029 - 9038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Adamson and S. Kenney The Epstein-Barr Virus BZLF1 Protein Interacts Physically and Functionally with the Histone Acetylase CREB-Binding Protein J. Virol., August 1, 1999; 73(8): 6551 - 6558. [Abstract] [Full Text]
|
|
|
|
|
|
A. Francis, T. Ragoczy, L. Gradoville, L. Heston, A. El-Guindy, Y. Endo, and G. Miller Amino Acid Substitutions Reveal Distinct Functions of Serine 186 of the ZEBRA Protein in Activation of Early Lytic Cycle Genes and Synergy with the Epstein-Barr Virus R Transactivator J. Virol., June 1, 1999; 73(6): 4543 - 4551. [Abstract] [Full Text]
|
|
|
|
|
|
H Gruffat, S Portes-Sentis, A Sergeant, and E Manet Kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) encodes a homologue of the Epstein-Barr virus bZip protein EB1 J. Gen. Virol., March 1, 1999; 80(3): 557 - 561. [Abstract]
|
|
|
|
|
|
V. L. Zacny, J. Wilson, and J. S. Pagano The Epstein-Barr Virus Immediate-Early Gene Product, BRLF1, Interacts with the Retinoblastoma Protein during the Viral Lytic Cycle J. Virol., October 1, 1998; 72(10): 8043 - 8051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Lehman, K. B. Ellwood, B. E. Middleton, and M. Carey Compensatory Energetic Relationships between Upstream Activators and the RNA Polymerase II General Transcription Machinery J. Biol. Chem., January 9, 1998; 273(2): 932 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cayrol and E. Flemington G0/G1 Growth Arrest Mediated by a Region Encompassing the Basic Leucine Zipper (bZIP) Domain of the Epstein-Barr Virus Transactivator Zta J. Biol. Chem., December 13, 1996; 271(50): 31799 - 31802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q Zhou, P M Lieberman, T G Boyer, and A J Berk Holo-TFIID supports transcriptional stimulation by diverse activators and from a TATA-less promoter. Genes & Dev., October 1, 1992; 6(10): 1964 - 1974. [Abstract] [PDF]
|
|
|
|
|
|
P M Lieberman and A J Berk The Zta trans-activator protein stabilizes TFIID association with promoter DNA by direct protein-protein interaction. Genes & Dev., December 1, 1991; 5(12b): 2441 - 2454. [Abstract] [PDF]
|
|