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Nucleic Acids Research, 1991, Vol. 19, No. 6 1285-1289
© 1991

MOLECULAR BIOLOGY

Antigenomic Hepatitis delta virus ribozymes self-cleave in 18 M formamide

Janet B. Smith and Gail Dinter-Gottlieb*

Department of Bioscience and Biotechnology, Drexel Universfty Philadelphia, PA 19104, USA

* To whom correspondence should be addressed

Received November 26, 1990. Revised February 4, 1991. Accepted February 4, 1991.

The ribozymes derived from Hepatitis defta virus (HDV) RNA appear unique in their sequence requirements for self-cleavage. While truncating the 1679 nucleotlde antlgenomic HDV RNA, we have characterized the cleavage requirements of a number of ribozymes of intermediate length. Two of these, containing 186 and 106 HDV nucleotides respectively, cleaved to completion in the presence of 18 M fomiamide. The 186 nucleotide ribozyme also cleaved to completion in 10 M urea. Removal of an additional 10 nts from the 3' terminus of the 106 nt ribozyme resulted in a loss of the ability to cleave in high concentrations of the denaturants. The interaction of nucleotides near the cleavage site with a sequence within this 10 base region may confer unusual stability on these ribozymes.


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