Skip Navigation

This Article
Right arrow Print PDF (429K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrowScopus Links
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zhang, T. Y.
Right arrow Articles by Cantor, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, T. Y.
Right arrow Articles by Cantor, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1991, Vol. 19, No. 6 1291-1296
© 1991

GENOME STRUCTURE AND MAPPING

Secondary pulsed field gel electrophoresis: a new method for faster separation of larger DNA molecules

Tai Yong Zhang1, Cassandra Smith1,2,* and Charles Cantor1,2

1Human Genome Center, Lawrence Berkeley Laboratory Berkeley, CA 94720, USA 2Department of Molecular and Cell Biology, University of California Berkeley, CA 94720, USA

* To whom correspondence should be addressed

Received November 7, 1990. Revised February 21, 1991. Accepted February 21, 1991.

A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 megabase pairs to over 10 megabase pairs in size. This Improved resolution is obtained with dramatically shorter run times. Thus SPFG appears to circumvent a number of the key limitations In previous PFG protocols.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.