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Nucleic Acids Research, 1991, Vol. 19, No. 7 1421-1426
© 1991

MOLECULAR BIOLOGY

Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1

Karola Rittner and George Sczakiel*

Institut für Virusforschung, Deutsches Krebsforschungszentrum Im Neuenheimer Feld 280, D-6900 Heidelberg, FRG

* To whom correspondence should be addressed

Received January 22, 1991. Revised February 28, 1991. Accepted February 28, 1991.

Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious pro viral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.


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