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Nucleic Acids Research, 1991, Vol. 19, No. 7 1443-1447
© 1991

MOLECULAR BIOLOGY

Effect of uracil situated in the vicinity of a mispair on the directionality of mismatch correction in Escherichia coli

Olga Aprelikova+ and Josef Jiricny*

Friedrich Miescher Institute PO Box 2543, CH-4002 Basel, Switzerland

* To whom correspondence should be addressed at IRBM, Via Pontina km 30,600, 00040, Pomezia, Italy

Received January 15, 1991. Revised March 11, 1991. Accepted March 11, 1991.

We wanted to establish whether strand breaks and gaps, arising during the removal of uracil from newly-synthesized DNA, can be utilized as strand discrimination signals by the methyl-directed mismatch repair system of Escherichia coli. For this purpose, we constructed a series of M13 heteroduplexes that contained a single uracil residue situated either upstream or downstream from a G/T or an A/C mispair. Transfections of these constructs into E. coli strains, either proficient of deficient in mismatch or uracil repair, allowed us to follow the fate of these mispairs in vivo. Our data show that the intermediates of uracil repair cannot substitute for the strand-discrimlnatlon signals generated by the MutH protein, which is thought to initiate the methyl-directed mismatch repair process by nicking the unmethylated strand of a newly-synthesized DNA duplex at d(GATC) sites. However, processing of uracil residues situated upstream from the mispair was shown to reduce the yield of the progeny phage arising from the uracil-containlng strand, presumably as a result of co-repair of the base analogue and the mispair.

+ Present address: Institute of Cytology, Tikhoretskii Av. 4, 194064 Lenningrad, USSR


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