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Nucleic Acids Research, 1991, Vol. 19, No. 8 1741-1744
© 1991

MOLECULAR BIOLOGY

An additional editing site is present in apolipoprotein B mRNA

Naveenan Navaratnam, Dipti Patel, Rajvee Shah, Jobst C. Greeve, Lyn M. Powell+, Timothy J. Knott§ and James Scott*

MRC Clinical Research Centre Watford Road, Harrow, Middlesex HA1 3UJ, UK

* To whom correspoadence should be addressed

Received February 13, 1991. Revised March 20, 1991. Accepted March 20, 1991.

Human intestinal apolipoprotein (apo) B mRNA undergoes a C to U RNA editing at nucleotlde 6666 to generate a translation stop at codon 2153, which defines the carboxy-terminal of apo B48. Here we show that two of eleven human intestinal cDNAs spanning residue 6666 were edited from a genomically-encoded C to a T at residue 6802 as well as at residue 6666. This additional editing converts Thr (ACA) codon 2198 to lie (AUA). Synthetic RNA including the nucleotide 6802 was edited in vitro by intestinal extracts at 10–15% of the editing efficiency of nucleotide 6666. A sequence is identif led as important for recognition by the editing activity. No secondary structural homology was identified between the two edited sites. No other sequence in the region between 6411 and 6893 nucieotides of apo B mRNA was found to be edited in vivo or in vitro. Apo B RNA editing extracts from intestine did not edit maize cytochrome oxldase II mRNA.

+Present addresses: Genentech Inc., 460 Point San Bruno Boulevard, South San Francisco, CA 94080, USA

§Present addresses: Amersham International plc, White Lion Road, Little Chalfont, Buckinghamshire HP7 9LL, UK


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