Nucleic Acids Research, 1991, Vol. 19, No. 8 1767-1771
© 1991
MOLECULAR BIOLOGY |
The 21 bp repeat element of the human cytomegalovirus major immediate early enhancer is a negative regulator of gene expression in undifferentiated cells
Department of Medicine, Universfty of Cambridge Addenbrooke's Hospital, Hills Road, Cambridge CB2 200, UK
* To whom correspondence should be addressed
Received January 28, 1991. Revised March 20, 1991. Accepted March 20, 1991.
The major immediate early regulatory region of human cytomegalovirus (HCMV) has a complex set of DNA sites through which both cellular and viral factors coordinately regulate immediate early gene expression. In undifferentiated human teratocarcinoma (T2) cells we have previously shown that major Immediate early gene expressIon is repressed by a differentiation specific nuclear factor MBF1, which binds to the Imperfect dyed symmetry located upstream of the enhancer. However, upon differentiation MBF1 decreases resulting In immediate early gene expression. In this study we show, by mobility shift analysis that the same or similar factor(s) also binds to the 2lbp repeat of the major immediate early enhancer. Deletion of this 2lbp repeat from the immediate early enhancer expression vectors results in increased CAT expression In undifferentiated T2 cells, to levels similar to that in differentiated cells. Consequently, the 21 bp repeat of the HCMV enhancer also acts to negatively regulate major immediate early enhancer function In non-permissive cells.
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