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Nucleic Acids Research, 1991, Vol. 19, No. 8 1811-1815
© 1991

MOLECULAR BIOLOGY

A method of identifying and isolating a unique member of a multigene family: application to a trypanosome surface antigen gene

Barbara J. Ruef, Jonathan H. Hecht and Jerry E. Manning*

Department of Molecular Biology and Biochemistry, University of California Irvine, CA 92717, USA

* To whom correspondence should be addressed

Received January 14, 1991. Accepted February 27, 1991.

A chimenc oligonucleotide was constructed using DNA sequences from two distal regions of a cDNA which encodes a major surface antigen (TSA-1) of Trypanosoma cruzi. Conditions were found that allowed the chimeric oligonucieotide to hybridize only to a 5.4 kb EcoR1 fragment in a Southern blot of total genomic DNA. The 5.4 kb EcoR1 genomic DNA fragment has previously been shown to be located at a telomeric site, thus the studies described here directly demonstrate that the TSA-1 gene is telomeric in location. It is also shown that the chlmeric oligonucleotide can be used to selectively identify recombinant {lambda} phage which harbor the TSA-1 gene using standard library screening procedures. Since these studies demonstrate that a chimeric oligonucleotide can be used to identify in both Southern blots and library screens a single member among the more than sixty members of the TSA-1 gene family, it seems likely that chimeric oligonucleotides may be of general use in studies involving repetitive DNA sequence families.


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