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Nucleic Acids Research, 1991, Vol. 19, No. 8 1879-1884
© 1991

MOLECULAR BIOLOGY

A simple method for quantifying specific mRNAs in small numbers of early mouse embryos

Michelle F. Gaudette and William R. Crain*

Cell Biology Group, Worcester Foundation for Experimental Biology 222 Maple Avenue, Shrewsbury, MA 01545, USA

* To whom correspondence should be addressed

Received November 14, 1990. Revised February 25, 1991. Accepted February 25, 1991.

Amplification of sequences by the polymerase chain reaction (PCR) has become a powerful tool in the study of gene expression. The technique is, in fact, so powerful that it may detect ‘leaky transcription’. Thus, it is now important to be able to quantify the transcripts that are amplified to determine whether or not they represent legitimate transcription of target genes. In this paper, we describe a one-step amplification reaction coupled to solution hybridizatlon/RNase protection that is capable of quantitating specific transcripts in total RNA from one to ten prelmplantation mouse embryos and is generally applicable to any cloned mRNA sequence.


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