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Nucleic Acids Research, 1991, Vol. 19, No. 8 1917-1924
© 1991

MOLECULAR BIOLOGY

The murine 2-5A synthetase locus: three distinct transcripts from two linked genes

Michael N. Ruthertord, Aseem Kumar, Ahuva Nissim1, Judith Chebath1 and Bryan R.G. Williams*

Research Institute, Hospital for Sick Children and Department of Molecular and Medical Genetics, University of Toronto Toronto M5G 1X8, Canada 1Department of Molecular Genetics and Virology, Weizniann lnstitute of Science Rehovot, Israel

* To whom correspondence should be addressed

Received October 19, 1990. Revised March 13, 1991. Accepted March 13, 1991.

The cloning of cDNAs encoding murine 2-5A synthetase has identified three related transcripts, represented by a previously described cDNA clone, L3 and two novel cDNAs, L1 and L2. L1 contains an open reading frame coding for a protein that shows 70% conservation at the amino acid level when compared to the protein predicted to be encoded by L3. L2 recognizes an IFN-induced transcript 600-bp larger than the L3 transcript. These three cDNAs map to a cosmid, cll, containing two murine 2–5A synthetase genes, ME12 and ME5/ME8, situated in a head-to-tail orientation separated by approximately 8 kb. Southern analyses of ME12 and ME5/ME8 using L3, L1-specific and L2-specific probes indicate that these genes have a similar organization. cll was translently and stably transfected into CV-1 cells. When treated with interferon, the transfected cells produced mature, murine 2–5A synthetase transcripts identified using L3 and L2-specific probes. Thus all transcripts present in IFN-treated mouse cells which are recognized by the available murine 2–5A synthetase cDNA probes map to an approximately 40 kb region of the mouse genome containing two 2–5A synthetase genes.


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