Nucleic Acids Research, 1991, Vol. 19, No. 9 2331-2335
© 1991
MOLECULAR BIOLOGY |
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes
Centre de Recherche en Cancérologie de I'Université Laval Hotel-Dieu de Québec, 11 Cote du Palais, G1R 2J6 Qúebec, Canada
*To whom correspondence should be addressed
Received February 7, 1991. Accepted March 27, 1991.
The RNA polymerase I transcription factor UBF has been Identified in human, mouse, rat and Xenopus and the primary structure of the human protein has been determined. Human UBF was shown to contain four tandem homologies to the folding domains of the HMG1 and 2 proteins and hence to belong to a previously unrecognised famllly of 'HMG-box' transcription factors. Here, cDNA clones encoding the Xenopus laevls UBF (xUBF) have been Isolated and sequenced. Northern and Southern blots revealed that In tissue culture cells, xUBF is coded on a single major mRNA size species by a small number of genes. The deduced primary structure of xUBF is highly homologous with the human protein except for a central deletion which removes most of one HMG-box. This explains the major size difference between the X. laevis and human proteins and may well explain their different transcriptlonal specificities. It Is shown that xUBF contains 5 tandemly repeated HMG-boxes and that by analogy the human protein contains 6.
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