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Nucleic Acids Research, 1975, Vol. 2, No. 1 101-112
© 1975

Articles

Transfer RNA methyltransferases from yellow lupin seeds: purification and properties

H. Wierzbicka, H. Jakubowski and J. Pawelkiewicz

Institute of Biochemistry, University of Agriculture Wolynska 35, 60-637 Poznan, Poland

Received November 29, 1974. tRNA methyltransferases from extract of yellow lupin seeds were purified over 300-fold by the methods based on hydrophobic and affinity chromatography. However, in the most active fractions the methylating enzymes were over 2000 purified. The purified enzyme fractions catalysed the formation of 1-methyladenine and 5-methylcytosine using E. coli B and B. subtilis tRNAs as substrates and Sadenosylmethionine as the methyl donor. They were unable to methylate their own endogenous tRNA but they were capable of methylating tRNA of some other Iupinus species. Whereas the patterns of methylated constituents of tRNA from E.coli and B. subtilis were quite similar, they differed considerably from those obtained with lupin species tRNAs. Some properties of purified methyltransferases from yellow lupin seeds have been described.


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