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Nucleic Acids Research, 1975, Vol. 2, No. 1 131-142
© 1975

Articles

Studies on the structure of replicative intermediates In bacteriophage M 13 single stranded DNA synthesis

Barbara E. Kessler-Liebscher, Walter L. Staudenbauer and Peter Hans Hofschneider

Max-Planck-Institut für Biochemie 8033 Martinsried bei München, Germany

Received December 9, 1974. Pulse-labeled replicative intermediates in M 13 single stranded DNA synthesis can be separated by dye-buoyant density centrifugation into two major fractions: Supercoiled molecules (RI I) containing viral strands of more than one genome length, and "relaxed" molecules (RI II) with labeled DNA chains shorter than unit length. It is postulated that RI II molecules might be formed in vivo by site-specific nicking of RF I molecules.


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