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Nucleic Acids Research, 1975, Vol. 2, No. 10 1727-1736
© 1975

Articles

The synthesis, of the internucleotide (phosphodiester) bond by a base-catalysed reaction*

Richard von Tigerstrom**, Patricia Jahnke and Michael Smith***

Department of Biochemistry, Faculty of Medicine, University of British Columbia Vancouver, B.C., Canada

Received July 8, 1975.

Potassium tert-butoxide in hexamethyl phosphoramide and dimethyl formamide provides an excellent catalyst for the reaction of a 3'-hydroxyl or a 5'-hydroxyl group of a nucleoside with an appropriate nucleoside phosphorofluoridate to yield the dinucleoside phosphate. This paper describes the experiments leading to the development of this reaction together with the synthesis of thymidylyl- (5' -> 3') -thymidine (dT-dT).

*Research supported by the Medical Research Council of Canada

**Department of Microbiology, University of Alberta, Edmonton, Alberta, Canada

***Medical Research Associate of the Medical Research Council of Canada


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