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Nucleic Acids Research, 1975, Vol. 2, No. 10 1771-1776
© 1975

Articles

A facile method for the preparation of N6-substituted ATP-Sepharose

F. Eckstein, M. Goumet and R. Wetzel

Max-Planck-Institut für experimentelle Medizin Abteilung Chemie, 3400 Göttingen, GFR

Received July 22, 1975. The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N6-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.


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