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Nucleic Acids Research, 1975, Vol. 2, No. 10 1931-1940
© 1975


Articles

Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's

Hiroshi Kasai, Yoshiyuki Kuchino, Kayoko Nihei and Susumu Nishimura

Biology Division, National Cancer Center Research Institute Tokyo, Japan

Received September 4, 1975. The modified nucleoside, 7-(4,5-cis-dihydroxy-l-cyclopenten-3-yl-aminomethyl)-7-deazaguanosine, designated as Q, and its derivative, Q*, were found in tRNA's from various organisms, including several mammalian tissues, other animals such as starfish, lingula and hagfish, and wheat germ.

Q isolated from rat liver tRNA was found to be identical with E. coli Q by mass spectrometry and thin-layer chromatography. Thus the rare modified nucleoside Q originally isolated from E. coli tRNA, is widely distributed in various organisms. Analysis of the mass spectrum of Q* suggested that it has a different side chain from Q.


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