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Nucleic Acids Research, 1975, Vol. 2, No. 10 1951-1966
© 1975

Articles

Application of fluorescamine to the study of protein-DNA interactions

Jürgen Bode and Lothar Willmitzer

Gesellschaft für Molekularbiologische Forschung, Abteilung Molekularbiologie D-3301 Stöckheim-Braunschweig, GFR

Received September 8, 1975. The reactivity of {alpha}-amino groups of basic proteins towards fluorescamine is essentially abolished if salt linkages with DNA phosphate groups are formed. This observation prompted the elaboration of a very general assay which allows the determination of binding parameters for the interaction of proteins with DNA and chromatin. Protamines, labeled with fluorescamine prior to their binding by DNA, appear to be useful probes to monitor the formation and nature of DNA-protein complexes.


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