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Nucleic Acids Research, 1975, Vol. 2, No. 10 1995-2006
© 1975

Articles

Mapping the transcription site of the SV40-specific late 16 S mRNA

E. May*, H. Kopecka** and P. May*

*Unite de Biophysique, Institut de Recherches Scientifiques sur le Cancer BP No. 8, 94 800-Villejuif, France **Laboratoire de Genetique Moleculaire, Institut de Biologie Moleculaire 2 Place Jussieu, 75 005-Paris, France

Received September 15, 1975. This paper describes the purification of polysomal RNA from SV4O-lytically infected CVl (monkey) cells and separation of the two distinct classes of SV4O-specific mRNA sedimenting at 16 S and 19 S. These classes have been hybridized with the whole SV4O DNA genome as well as with the SV40 Hind fragments. The results have permitted the mapping of SV40-specific late 16 S mRNA from approximately 0.945 to 0.175 map units.


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